| Branch Name | First Stop | Start Time | Last Stop | End Time | Travel Time |
|---|
|
| ↑16 - Union Station | Hamilton GO Centre Bus | 5:15:00AM | Union Station Bus Terminal | 6:15:00AM | 1:00:00 |
| ↓16 - Hamilton Go | Union Station Bus Terminal | 5:30:00AM | Hamilton GO Centre Bus | 6:25:00AM | 55:00 |
| ↓16 - Hamilton Go | Union Station Bus Terminal | 6:30:00AM | Hamilton GO Centre Bus | 7:30:00AM | 1:00:00 |
| ↑16 - Union Station | Hamilton GO Centre Bus | 8:55:00AM | Union Station Bus Terminal | 10:15:00AM | 1:20:00 |
| ↑16 - Union Station | Hamilton GO Centre Bus | 9:55:00AM | Union Station Bus Terminal | 11:10:00AM | 1:15:00 |
| ↓16 - Hamilton Go | Union Station Bus Terminal | 10:30:00AM | Hamilton GO Centre Bus | 11:40:00AM | 1:10:00 |
| ↑16 - Union Station | Hamilton GO Centre Bus | 10:55:00AM | Union Station Bus Terminal | 12:10:00PM | 1:15:00 |
| ↓16 - Hamilton Go | Union Station Bus Terminal | 11:30:00AM | Hamilton GO Centre Bus | 12:45:00PM | 1:15:00 |
| ↑16 - Union Station | Hamilton GO Centre Bus | 11:50:00AM | Union Station Bus Terminal | 1:05:00PM | 1:15:00 |
| ↓16 - Hamilton Go | Union Station Bus Terminal | 12:30:00PM | Hamilton GO Centre Bus | 1:45:00PM | 1:15:00 |
| ↑16 - Union Station | Hamilton GO Centre Bus | 12:55:00PM | Union Station Bus Terminal | 2:10:00PM | 1:15:00 |
| ↓16 - Hamilton Go | Union Station Bus Terminal | 1:30:00PM | Hamilton GO Centre Bus | 2:50:00PM | 1:20:00 |
| ↓16 - Hamilton Go | Union Station Bus Terminal | 2:30:00PM | Hamilton GO Centre Bus | 4:10:00PM | 1:40:00 |
| ↑16 - Union Station | Hamilton GO Centre Bus | 5:45:00PM | Union Station Bus Terminal | 7:15:00PM | 1:30:00 |
| ↑16 - Union Station | Hamilton GO Centre Bus | 6:55:00PM | Union Station Bus Terminal | 8:05:00PM | 1:10:00 |
| ↓16 - Hamilton Go | Union Station Bus Terminal | 7:35:00PM | Hamilton GO Centre Bus | 8:45:00PM | 1:10:00 |
| ↑16 - Union Station | Hamilton GO Centre Bus | 8:00:00PM | Union Station Bus Terminal | 9:00:00PM | 1:00:00 |
| ↓16 - Hamilton Go | Union Station Bus Terminal | 8:30:00PM | Hamilton GO Centre Bus | 9:35:00PM | 1:05:00 |
| ↑16 - Union Station | Hamilton GO Centre Bus | 8:55:00PM | Union Station Bus Terminal | 9:55:00PM | 1:00:00 |
| ↓16 - Hamilton Go | Union Station Bus Terminal | 9:30:00PM | Hamilton GO Centre Bus | 10:35:00PM | 1:05:00 |
| ↑16 - Union Station | Hamilton GO Centre Bus | 9:55:00PM | Union Station Bus Terminal | 10:55:00PM | 1:00:00 |
| ↓16 - Hamilton Go | Union Station Bus Terminal | 10:30:00PM | Hamilton GO Centre Bus | 11:30:00PM | 1:00:00 |
| ↑16 - Union Station | Hamilton GO Centre Bus | 10:55:00PM | Union Station Bus Terminal | 11:55:00PM | 1:00:00 |
| ↓16 - Hamilton Go | Union Station Bus Terminal | 11:30:00PM | Hamilton GO Centre Bus | 12:30:00AM | 1:00:00 |
| ↓16 - Hamilton Go | Union Station Bus Terminal | 12:30:00AM (following day) | Hamilton GO Centre Bus | 1:25:00AM | 55:00 |
| ↑16 - Union Station | Hamilton GO Centre Bus | 1:00:00AM (following day) | Union Station Bus Terminal | 1:55:00AM | 55:00 |